Molecular Biology Principles and Practices

MOLECULAR BIOLOGY (Principles and Practices) introduces undergraduates, postgraduates as well as research students of biotechnology to basic experimental molecular biology laboratory techniques. Emphasis has been given to techniques encountered in practical classes with the principles and theories behind this explained in detail. No contemporary book on Practical Molecular Biology would be complete without chapters on Bio-Safety, Laboratory Set-up, Molecular Biology Calculations and Glossary. These topics have been extensively covered in this book. This is essential reading for all biosciences students, biotechnology and preclinical medical students for whom practical molecular biology forms part of the syllabus.





UNIT I : MOLECULAR BIOLOGY LABORATORY-AN INTRODUCTION

Part 1 - General Laboratory Facilities

Part 2 - General Laboratory Practices

Part 3 - Laboratory Safety Guidelines and Health Hazards

UNIT II : CALCULATIONS USED IN MOLECULAR BIOLOGY

Part 1 - Calculations used in Molecular Biology

UNIT III : DNA ISOLATION AND PURIFICATION

Part 1 - Plasmid DNA

Part 2 - Bacterial (E. Coli) DNA

Part 3 - Plant Genomic DNA

Part 4 - Animal Genomic DNA

UNIT IV : ELECTROPHORESIS

Part 1 - A General Account

Part 2 - Agarose Gel Electrophoresis of DNA

Part 3 - Polyacrylamide Gel Electrophoresis of DNA

Part 4 - Polyacrylamide Gel Electrophoresis of Proteins

UNIT V : CHECKING QUALITY AND QUANTITY OF DNA

Part 1 - Spectrophotometric Method

Part 2 - Electrophoretic Method

UNIT VI : RESTRICTION ENDONUCLEASES

Part 1 - A General Account

Part 2 - Restriction Enzyme Digestion and Size Determination of DNA Fragments

Part 3 - Restriction Mapping of Lambda DNA with three Enzymes viz Eco RI, Bam HI and Hind III

UNIT VII : LIGATION

Part 1 - A General Account

Part 2 - Ligation of a DNA Fragment, Carrying Gene of Interest, to a Plasmid for Constructing a Recombinant DNA Molecule

UNIT VIII : TRANSFORMATION

Part 1 - Transformation a General Account

Part 2 - Bacterial Plasmid Transformation

Part 3 - Agrobacterium-Mediated Transformation in Plants

(e.g., Nicotiana Tabacum)

UNIT IX : POLYMERASE CHAIN REACTION

Part 1 - PCR-a General Account

Part 2 - Performing Amplification of DNA using PCR in a Routine Laboratory Experiment

UNIT X : MOLECULAR MARKERS

Part 1 - Molecular Markers-a General Account

Part 2 - RFLP (Restriction Fragment Length Polymorphism)

Part 3 - RAPD (Random amplified polymorphic DNA)

Part 4 - AFLP (Amplified Fragment Length Polymorphism)

Part 5 - Simple Sequence Repeats (SSRs) or Micro Satellites

UNIT XI : MOLECULAR MARKERS AND MAPPING

Part 1 - Molecular Markers and Mapping

UNIT XII : DNA SEQUENCING

Part 1 - A General Account

Part 2 - Performing DNA Sequencing in Laboratory by the Sanger's Dideoxy Method using Sequenase Enzyme

UNIT XIII : IN VITRO MUTAGENESIS

Part 1 - A General Account

Part 2 - Performing Oligonucleotide Directed Mutagenesis of Single Stranded DNA